5x HOT FIREPol® Probe Universal qPCR Mix, 250 reactions

08-88-002 0

Optimised for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. Login to view prices and add products to cart.

x HOT FIREPol® Probe Universal qPCR Mix is optimised for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables efficient amplification of AT-rich, regular and GC-rich (up to 7 %) targets. HOT FIREPol® Probe Universal qPCR Mix is optimized for DNA/LNA hydrolysis probes based on the ′ flap endonuclease activity.

HOT FIREPol® DNA Polymerase is activated by a 10 min incubation step at 9 °C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup. Features & Benefits<....